Journal: International Journal of Molecular Sciences
Article Title: The Effect of miR-140-5p with HDAC4 towards Growth and Differentiation Signaling of Chondrocytes in Thiram-Induced Tibial Dyschondroplasia
doi: 10.3390/ijms241310975
Figure Lengend Snippet: The proteins expression level through Western blotting. ( A ): Analysis of expression levels of marker proteins (Col-II, Sox9, PTHrP, and HDAC4) in chondrocytes at different stages (growth and differentiation), using β-actin and GAPDH as internal reference proteins. ( B ): Analysis of expression levels of marker proteins (MEF2C, Runx2, Col-X, MMP-13, IHH, COMP, and VEGFA) in chondrocytes at different stages (growth and differentiation), using β-actin and GAPDH as internal reference proteins. The red text refers to a distinct band corresponding to targeted molecular weight (MW). “*” shows the level of significance among groups. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: The following antibodies were used: rabbit anti-COL2A1 (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A1560), rabbit anti-Aggrecan (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A8536), rabbit anti-IHH (1:1500 for WB, ABclonal, A6626), rabbit anti-SOX9 (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A2479), rabbit anti-PTHLH (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A12492), rabbit anti-HDAC4 (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A0179), rabbit anti-HDAC4 (1:100 for IP, ABclonal, A0239), rabbit anti-CMP (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A13963), rabbit anti-VEGF (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A12303), rabbit anti-RUNX2 (1:1000 for WB, 1:200 for IHC/IF, ABclonal, A2851), rabbit anti-MEF2C (1:1500 for WB, 1:100 for IHC/IF, ABclonal, A12385), rabbit anti-MMP13 (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A11148), rabbit anti-Collagen X/COL10A1 (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A18604), rabbit anti-BMP2 (1:1500 for WB, 1:200 for IHC/IF, ABclonal, A0231), rabbit anti-GAPDH (1:5000 for WB, 1:00 for IHC/IF, 1:50 for IP, ABclonal, AC001), rabbit anti-β-Actin (1:10,000 for WB, ABclonal, AC026), HRP Goat Anti-Rabbit IgG (H + L) (1:5000 for WB, ABclonal, AS014), Fluorescein(FITC)-conjugated Affinipure Goat Anti- Rabbit IgG (H + L) (1:100 for IF, proteintech, SA00003-2), Cy3-conjugated Affinipure Goat Anti- Rabbit IgG (H + L) (1:100 for IF, proteintech, SA00009-2).
Techniques: Expressing, Western Blot, Marker, Molecular Weight
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